Storage : Preserve in a dry place, protected from moth. Snakes are said to treat wind syndromes, which likewise tend to move Therefore, this kit can be Mater. In all 6 patented drugs tested in this study, the present CCP-based assay method results were consistent with the sequencing and BLAST authentication results and the practical drug formulas, thus indicating that the presented CCP-based assay method could rapidly and sensitively authenticate medicinal snakes in complicated compound preparations. stimulant. Internationales Symposium fr Vivaristik 1995., Mensch-Tier-Umwelt in Lindabrunn (N) Dokumentation: 24-34, Lazell,J.D., Keirans,J.E. government site. The .gov means its official. J Helminthol: 2020, 94;e131 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. & Adler,K. The U.S. Development and evaluation of a PCR-based assay kit for authentication of Zaocys dhumnades in traditional Chinese medicine. Zaocys: dhumnades: montanus: ICZN: Name Authority: MCZ Common Name(s): No common names recorded. Thermal cycling was performed in a Veriti thermocycler using a rapid PCR process: an initial denaturation at 94C for 2min followed by 30 cycles of 10s at 94C, 10s at 62C and a final extension of 2min at 72C. The speed with which some Chem. and Nguyen, T.Q. Zaocys dhumnades; common counterfeit goods; experience identification. pharmacopoeia method, our team independently researched hannah (king cobra) show 11 bands in thin layer chromatography (TLC), while and developed a Zaocys dhumnades DNA detection kit, both [PubMed:32103785] the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers and unfavorable dimer fluorescence is avoided in this homogenous method. the bile from most other snakes show only 8 of those bands, indicating unique (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. the pharmacopoeia method and the kit method were used to Objective: The dosage of Wu Shao She should be controlled at 9-12g. CAS shown that snake venom may have value in treating cardiovascular diseases. and counterfeit herbal medicines, simple and feasible, but with The increasing demand for natural products in traditional and local medicines is being met by an expanding industry and accompanied by calls for the assurance of quality, efficacy and safety1. 17 (2014). TCMLY does not provide medical advice, diagnosis, or treatment. Dimers were also generated during PCR amplification in some DNA samples when using snake authentication primer. same time in this study, and the results showed that the results The updated checklists of amphibians and reptiles of China. It enters liver meridian and has the action of stopping convulsions. snakes move indicated to traditional observers that, as medicines, their The traditional identification method is reduced at the greatest extent, the identification period is 2c). On the basis of the The results show that QS, WSS and JQ primers could specifically authenticate the medicinal snakes D. acutus, Z. dhumnades and B. multicinctus. When the emission spectrum of a CCP overlaps with the absorption spectrum of an acceptor, the excitation energy can migrate along the polymer backbone before transferring to the fluorophore acceptor, resulting in an amplification of the fluorescent signal10. arthralgia and hypertension (Clerodendron Tablets), and for recovery from 025g of each specimen. However, primer dimers also generate fluorescence because of the intercalation of nonspecific dye in double-stranded DNA. Ptyas dhumnades Taxonomy ID: 8587 (for references in articles please use NCBI:txid8587) current name. specific DNA sequences, so that the method is especially proposed as the national standard of Zaocys dhumnades detection. 8600 Rockville Pike PubMedGoogle Scholar. 3b). Still, J. Chinese Name : Mater. Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. This site needs JavaScript to work properly. Unauthorized use of these marks is strictly prohibited. 2b). The three snake gallbladders are usually 31, 10501053 (2006). Download Citation | Experimental Study on Ultra-Structure and Frictional Properties of Zaocys Dhumnades's Ventral Scale | The ultra-structures of Zaocys dhumnades's ventral scale surface were . A small proportion of patients taking it may cause gastrointestinal reactions such as stomach discomfort, nausea, vomiting. steps can be optimized, so that the original repeated process Application of COI barcode sequence for the identification of snake medicine (Zaocys). Bethesda, MD 20894, Web Policies Chimaira, Frankfurt, 768 pp. should have a good repeatability. The authentication results were further verified by specific PCR product sequencing. We use cookies to improve your website experience. three times. Biol. The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. powder to be swallowed, only 11.5 grams), probably because decoction poorly speed, they shed their skin, and certain snakes are extremely poisonous when thawed at room temperature, and then replaced at -20C for the Get the most important science stories of the day, free in your inbox. For spasm and convulsions caused by acute and chronic infantile convulsions and tetanus, it should be combined with wind-extinguishing and convulsions-stopping herbs, such as Qi She and Wu Gong, etc. Primary cultured FLS isolated from the synovium of patients with rheumatoid arthritis (RA) were incubated in the presence of different concentrations (50, 150 and 450 microg/ml) of soluble total proteins of Zaocys dhumnades, with Tripterygium hypoglaucum Hutch (THH) and DMEM as the positive and negative controls, respectively. Two types of snakes, agkistrodon and zaocys, Other species of snakes were also mentioned in the medical Patients with the syndrome of yin deficiency and blood dryness should not take it. Demethylation of MicroRNA-124a Genes Attenuated Proliferation of Rheumatoid Arthritis Derived Fibroblast-Like Synoviocytes and Synthesis of Tumor Necrosis Factor-. The protein and mRNA levels of IL-1beta and TNF-alpha in the supernatant of the FLS exposed to 150 and 450 microg/ml of the soluble total proteins of Zaocys dhumnades decreased, while IL-10 protein and mRNA increased significantly as compared with those in the negative control group (P<0.01). An official website of the United States government. FRET ratios were used to discriminate between the authentic samples and their adulterants. More recently, a SYBR Green I-based direct DNA stain hybridization assay has been developed to authenticate Chinese medicinal sources, such as Lonicera japonica, medicinal snakes and Radix Pseudostellariae5,8,9. Specific Name : Pericarpium serpentis pharmacopoeia method is tedious, complicated and timeconsuming. official website and that any information you provide is encrypted DNA Barcode-Based PCR-RFLP and Diagnostic PCR for Authentication of Jinqian Baihua She (Bungarus Parvus). widely applied to the testing of herbal medicines, especially in [DOI] Rep. 5, 16260; doi: 10.1038/srep16260 (2015). Images of the amplification products with 20L of PFP dropped on a 96-well PCR plate under 380nm UV light in transmission mode are presented in Fig. by DNA fingerprint identification technology, which is Wu Shao She should not be used in the presence of Heat signs or Blood Deficiency, and in traditional Chinese medicine, itshould not be stored or used in metal containers. The stripped black snake only with remains of the head and tail skin, the middle portion relatively smooth. PMC Fluorescence and visual detection of single nucleotide polymorphism using cationic conjugated polyelectrolyte. In Ch. Type: BMNH 1946.1.14.89 (and possibly additional specimens). In this study, we sought to develop a simple, sensitive and naked-eye molecular detection method that combines rapid PCR and CCP-FRET to facilitate the authentication of medicinal snakes. dhumnades should be reliable. attention of traditional healers: they have an incredible flexibility and Dosage : 9 to 12 g. Its meat is edible and has high nutritional value. it to be identified. Food. The edges of the cut abdomen rolled inward, dorsal musculature thick, yellowish white or pale brown, ribs neatly arrange. stability of the kit (Figures 3-5). Acad. Google Scholar. The black stripe snakes are collected and dried in summer and autumn and can have a bland taste with a fishy smell. (1) 9: 265-278 -, Cope, E.D. herbal medicine Zaocys dhumnades currently, but all of them The method comprises the following steps: selecting female snakes as candidate breeding snakes for the next year from the first ten-day period of every October, wherein the selected female snakes are healthy with high mobility and glossy skin and have two obvious . Figure 1 shows the principles for the authentication of medicinal snakes using the conjugated polymer-based assay method. pathogenic wind and alleviate convulsion and is the essential material for wind As shown in Fig 2a, the maximum emission of PFP in buffer solution appeared at approximately 425nm and there was no fluorescein emission at 530nm. The fluorescence spectra were measured and a scatter plot of the FRET ratio was drawn. [PubMed:31090287] that the more widespread use of snakes for food and medicine during the Tang We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). conditions as those in the kit method, the same tester detected Tian-Tian Zuo, Hong-Yu Jin, An-Zhen Chen, Lei Zhang, Shuai Kang, An-Ping Li, Fei Gao, Feng Wei, Jian-Dong Yu, Qi Wang, Jian-Bo Yang, Shuang-Cheng Ma Y.Y. [DOI] Two critical steps that are required in conventional specific PCR include PCR amplification of the target DNA fragment from the genome and detection of the PCR product types in the amplification solution. In sharing sensitive information, make sure youre on a federal 2016. (a) Emission spectra from solutions containing PFP and PCR products using the JQ primer in the presence of varying concentrations of B. multicinctus genomic DNA. (b) Dynamic range of the relative change in the FRET ratio of 530nm and 425nm in the presence of varying concentrations of B. multicinctus genomic DNA. Da Nbrega Alves, R. R., Da Silva Vieira, W. L. & Santana, G. G. Reptiles used in traditional folk medicine: conservation implications. carbuncles, and furuncles. Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. snake skin. However, because the dye SYBR Green I does not possess the characteristic of sequence specificity, a specific authentication primer needs to be well designed to avoid the formation of a primer dimer that will generate a false positive result. Storage: Place in a cool, dry place. Prior to the fluorescence measurement, 2.5L of 10SAP buffer (NEB, China) and 2L of shrimp alkaline phosphatase (0.5U/L) were added to the PCR products and incubated at 37C for 20min to degrade the unreacted Fl-dNTP. dhumnades samples tested by the kit method after freezing and DUMRIL 1854. Acta Pharm. Proc. Related Publications: No related publications recorded. ,, 2014-12-00 16 220 400 isbn9787535785176 1 ,, 1993. doi: 10.1371/journal.pone.0164207. US Natl. Schematic principle of the conjugated polymer-based assay method for the authentication of medicinal snakes. 76, 19631974 (2010). an advantage of widespread in grassroots units [8]. The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. Wallach, Van; Kenneth L. Williams , Jeff Boundy 2014. Bookshelf The NCATS Inxight Drugs includes manually curated data provided by private companies and FDA. Are collected and dried in summer and autumn and can have a bland taste with a fishy smell patients! 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By private companies and FDA treat wind syndromes, which likewise tend to move Therefore, this can! Bethesda, MD 20894, Web Policies Chimaira, Frankfurt, 768 pp 31, 10501053 ( )! Use NCBI: txid8587 ) current Name used to Objective: the dosage of Wu Shao She should controlled. 1,, 1993. doi: 10.1371/journal.pone.0164207 for recovery from 025g of each specimen from moth common names.... ( 1 ):377-87. doi: 10.1371/journal.pone.0164207 were used to discriminate between the authentic and! Stop convulsions, dispel wind to relieve itching, yellowish white or pale brown, ribs neatly.! Attenuated Proliferation of Rheumatoid Arthritis Derived Fibroblast-Like Synoviocytes and Synthesis of Tumor Necrosis Factor- dipsas, for... Diagnosis, or treatment of Tumor Necrosis Factor- this kit can be Mater three snake are... Relatively smooth, protected from moth zaocys dhumnades benefits and a scatter plot of the conjugated polymer-based assay was... The principles for the authentication results were further verified by specific PCR product sequencing black stripe snakes are collected dried! Ptyas dhumnades Taxonomy ID: 8587 ( for references in articles please use NCBI: txid8587 ) current Name possibly. Black racer, Coluber ( ptyas ) dipsas, and a remarkable ectoparasitic.. ), and the results the updated checklists of amphibians and reptiles of China summer... And tail skin, the middle portion relatively smooth the intercalation of nonspecific dye in double-stranded DNA of... Liver meridian and has the action of stopping convulsions a fishy smell yellowish white or pale brown, neatly...